mouse anti psd95 primary antibody  (Fisher Scientific)

Journal: Cell reports

Article Title: Activity-dependent synthesis of Emerin gates neuronal plasticity by regulating proteostasis

doi: 10.1016/j.celrep.2025.115439

Figure Lengend Snippet: (A–H) Synaptic density analysis with Emerin KD and OE. (A and B) Experimental workflow (A). DIV12 neuronal cultures were immunolabeled with synaptic markers (B). (C) Color-code for data in (E) and (H). (D) Representative images of Synaptophysin and PSD95 immunolabeling with Emerin KD (2 top rows) and Emerin OE in excitatory (2 center rows) and inhibitory neurons (2 bottom rows). (E) Quantification of Synaptophysin and PSD95 puncta and their colocalization with Emerin KD (top row), Emerin OE in excitatory (center row), and inhibitory neurons (bottom row). (F and G) Representative images of Homer1 and Gephyrin with Emerin KD (F) and Emerin OE in excitatory neurons (G). Boxed regions in DAPI images are shown enlarged for Homer1 and Gephyrin. (H) Quantification of Homer1 and Gephyrin puncta with Emerin KD (top row) and Emerin OE in excitatory (center row) and inhibitory neurons (bottom row). (E and H) Quantification of postsynaptic components is highlighted in gray. Punctum counts are normalized to neuron numbers, based on DAPI. n = 12 individual wells from 4 independent culture preparations, Mann-Whitney test. Lines indicate median and 95% CI. (I–N) Spine morphology analysis with Emerin KD. (I) Representative image of a sparsely labeled neuron expressing EGFP. Blue rectangle, a representative secondary dendrite selected for analysis. (J) Representative dendritic stretches from Emerin KD and sham KD neurons. Examples of the four spine types quantified are labeled. (K–N) Counts of all spine types (K), filopodia (L), mushroom (M), and all types excluding filopodia (N). n = 10–11 ~45-mm dendritic stretches, each from one individual neuron. 3 independent culture preparations, Mann-Whitney test. Lines indicate median and 95% CI. Scale bars: 50 μm (D), 100 μm (I), and 10 μm (J). The scale bars in (G) (50 μm) apply to (F).

Article Snippet: Mouse anti-PSD95 monoclonal primary antibody , Cell signaling #36233 , RRID: AB_2721262.

Techniques: Immunolabeling, MANN-WHITNEY, Labeling, Expressing

Journal: Journal of Neuroinflammation

Article Title: Biologic TNF-α inhibitors reduce microgliosis, neuronal loss, and tau phosphorylation in a transgenic mouse model of tauopathy

doi: 10.1186/s12974-021-02332-7

Figure Lengend Snippet: Effect of TNF-α inhibitors on neuronal health. Representative images of H&E-stained hippocampal granule cell layer of the dentate gyrus (DG) in the PS19 mice ( A ). Scale bar = 200 μm. The thumbnail images showing the DG regions that were imaged are taken and adapted from the Allen Institute. The DG granule cell layer area, as measured using ImageJ, was significantly greater in the WT-Saline, PS19-TfRMAb-TNFR, and PS19-Etanercept mice compared to PS19-Saline controls ( B ). Scatter plot showing that AT8-positive area (%) was negatively correlated with the total hippocampal neuronal cell area ( C ; Pearson r = -0.42, p < 0.05). PSD95 (synaptic/neuronal health marker) protein level in the whole-brain homogenates detected by Western blotting was significantly increased in the PS19-TfRMAb-TNFR and PS19-Etanercept mice compared to PS19-Saline controls ( D ). Data are presented as mean ± SEM of 6–11 mice per treatment group (3–7 per sex per group). For H&E staining, n = 6 mice per treatment group. For the PSD95 Western, n = 7–11 mice per treatment group. Male and female mice were combined due to a lack of sex-related effects. One-way ANOVA with Holm–Sidak’s post hoc test was used to compare to PS19-Saline controls. * p < 0.05, *** p < 0.001, and **** p < 0.0001

Article Snippet: Membranes were sequentially subjected to blocking in Tris-buffered saline (TBS) containing 5% non-fat milk for 1 h at room temperature and probed with the mouse anti-PSD95 primary antibody (a marker of synaptic/neuronal health) (1:1000 in 3% non-fat milk, Santa Cruz Biotechnology, TX, USA) overnight at 4 °C followed by washing with TBS with 0.1% Tween-20 (TBST).

Techniques: Staining, Saline, Marker, Western Blot